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작성자 Ashely 작성일24-05-17 04:10 조회20회 댓글0건관련링크
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이름 : Ashely
이메일 : ashelywetherspoon@uol.com.br 연락처 : 예식일 : Array GE array Rai stage 0 0 0 I I II II 0 II 0 II 문의내용: Array Marimastat GE array Rai stage 0 0 0 I I II II 0 II 0 II II II I I II I I I 0 II II I I II II Age 41 63 69 45 60 50 59 57 65 61 65 67 48 58 46 59 40 67 51 57 65 52 60 44 68 80 Tumor Percentage 67.2 69 88.6 95.4 93.5 66 97.2 95.3 92.8 69.3 97.2 98 92 95.62 79.4 73 90 79.3 80 72.5 68.6 66 92 85.1 93 68.4 IGHV status UM UM UM UM UM UM M M M M M UM UM UM UM UM UM UM M M M M M M M M 2M(mg/L) 6.56 4.06 8.42 4.6 3.37 4.78 3.54 6.49 4.58 3.3 4.48 5.69 4.42 6.31 6.78 4.43 7.74 2.59 5.44 2.91 7.1 3.2 6.56 3.62 6.47 6.46 17p Deletion Absent Absent Absent Present Absent Absent Absent Absent Absent Absent Present Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent IPI Score 4 4 5 9 3 5 3 2 3 0 7 6 5 5 5 5 5 4 3 0 2 1 3 3 4Abbreviations used: GE Gene expression, UM Unmutated, M Mutated , 2 M Beta 2 Microglobulin, IPI International Prognostic indexLouis, MO, USA), labelled with Cy3- and Cy5-dUTP, respectively, and hybridized on 1x244K human promoter chIP-on-chip microarray slides as per the manufacturer's recommendations (Agilent Technologies, Santa Clara, CA, USA). The slides PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22993420 were washed and scanned on the Agilent DNA microarray scanner D and the data was extracted with Feature Extraction?software FE version 11.5 (Agilent Technologies, Santa Clara, CA, USA).Gene expression microarrayUSA). The slides were washed and scanned on the Agilent DNA microarray scanner D and the data was extracted with Feature Extraction?software FE version 11.5 (Agilent Technologies, Santa Clara, CA, USA). These samples included seven CLL samples profiled for DNA methylation status.Bisulfite genome sequencingTotal RNA obtained from PBMC of CLL patients (n = 21) and CD19+ sorted cells pooled from 10 healthy individuals was amplified and simultaneously labelled with Cy3CTP using low input quick amp labelling kit (Agilent Technologies, Santa Clara, CA, USA). The labelled product was finally hybridized to SurePrint G3 Human Gene Expression 8x60K microarray slide as per manufacturer's recommendation (Agilent Technologies, Santa Clara, CA,Genomic DNA (2 g) was bisulfite modified and purified using Epitect Bisulfite kit as per the manufacturer's instructions (Qiagen, Hilden , Germany). The bisulfite converted DNA was amplified for two CpG islands in PAX9 gene as depicted in Fig. 1 and sequenced with BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, CA, USA) with primers designed using MethPrimer (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi). The percent methylation levels were computed and further PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9221828 analysed with BisulfiteRani et al. Clinical Epigenetics (2017) 9:Page 4 ofFig. 1 Location of CpG islands studied for PAX9 gene methylation. a UCSC browser view of PAX9 gene (chromosome 14q13.3). The probes used for methylation microarrays were specific for CpG islands 121, 129, 39, and 76. b MethPrimer based CpG prediction and primer design for bisulfite gene sequencing for two CpG islands (3 and 7) located at PAX9 upstream region. c Bisulfite sequencing of CpG islands 3 and 7 was performed in 21 and 23 CLL patients respectively and in five healthy controls. A representative electropherogram depicting two methylated (C) or unmethylated (T) CpG sites in island 3 located in 5' region of PAX9 is shownSequencing DNA Methylation Analysis (BISMA) software (http://services.ibc.uni-stuttgart.de/BDPC/BISMA/).Real-time quantitative PCR (RQ-PCR)The mRNA expression based microarray findings.
이메일 : ashelywetherspoon@uol.com.br 연락처 : 예식일 : Array GE array Rai stage 0 0 0 I I II II 0 II 0 II 문의내용: Array Marimastat GE array Rai stage 0 0 0 I I II II 0 II 0 II II II I I II I I I 0 II II I I II II Age 41 63 69 45 60 50 59 57 65 61 65 67 48 58 46 59 40 67 51 57 65 52 60 44 68 80 Tumor Percentage 67.2 69 88.6 95.4 93.5 66 97.2 95.3 92.8 69.3 97.2 98 92 95.62 79.4 73 90 79.3 80 72.5 68.6 66 92 85.1 93 68.4 IGHV status UM UM UM UM UM UM M M M M M UM UM UM UM UM UM UM M M M M M M M M 2M(mg/L) 6.56 4.06 8.42 4.6 3.37 4.78 3.54 6.49 4.58 3.3 4.48 5.69 4.42 6.31 6.78 4.43 7.74 2.59 5.44 2.91 7.1 3.2 6.56 3.62 6.47 6.46 17p Deletion Absent Absent Absent Present Absent Absent Absent Absent Absent Absent Present Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent Absent IPI Score 4 4 5 9 3 5 3 2 3 0 7 6 5 5 5 5 5 4 3 0 2 1 3 3 4Abbreviations used: GE Gene expression, UM Unmutated, M Mutated , 2 M Beta 2 Microglobulin, IPI International Prognostic indexLouis, MO, USA), labelled with Cy3- and Cy5-dUTP, respectively, and hybridized on 1x244K human promoter chIP-on-chip microarray slides as per the manufacturer's recommendations (Agilent Technologies, Santa Clara, CA, USA). The slides PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22993420 were washed and scanned on the Agilent DNA microarray scanner D and the data was extracted with Feature Extraction?software FE version 11.5 (Agilent Technologies, Santa Clara, CA, USA).Gene expression microarrayUSA). The slides were washed and scanned on the Agilent DNA microarray scanner D and the data was extracted with Feature Extraction?software FE version 11.5 (Agilent Technologies, Santa Clara, CA, USA). These samples included seven CLL samples profiled for DNA methylation status.Bisulfite genome sequencingTotal RNA obtained from PBMC of CLL patients (n = 21) and CD19+ sorted cells pooled from 10 healthy individuals was amplified and simultaneously labelled with Cy3CTP using low input quick amp labelling kit (Agilent Technologies, Santa Clara, CA, USA). The labelled product was finally hybridized to SurePrint G3 Human Gene Expression 8x60K microarray slide as per manufacturer's recommendation (Agilent Technologies, Santa Clara, CA,Genomic DNA (2 g) was bisulfite modified and purified using Epitect Bisulfite kit as per the manufacturer's instructions (Qiagen, Hilden , Germany). The bisulfite converted DNA was amplified for two CpG islands in PAX9 gene as depicted in Fig. 1 and sequenced with BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, CA, USA) with primers designed using MethPrimer (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi). The percent methylation levels were computed and further PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9221828 analysed with BisulfiteRani et al. Clinical Epigenetics (2017) 9:Page 4 ofFig. 1 Location of CpG islands studied for PAX9 gene methylation. a UCSC browser view of PAX9 gene (chromosome 14q13.3). The probes used for methylation microarrays were specific for CpG islands 121, 129, 39, and 76. b MethPrimer based CpG prediction and primer design for bisulfite gene sequencing for two CpG islands (3 and 7) located at PAX9 upstream region. c Bisulfite sequencing of CpG islands 3 and 7 was performed in 21 and 23 CLL patients respectively and in five healthy controls. A representative electropherogram depicting two methylated (C) or unmethylated (T) CpG sites in island 3 located in 5' region of PAX9 is shownSequencing DNA Methylation Analysis (BISMA) software (http://services.ibc.uni-stuttgart.de/BDPC/BISMA/).Real-time quantitative PCR (RQ-PCR)The mRNA expression based microarray findings.
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